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KMID : 0604520150410030287
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Journal of the Society of Cosmetic Scientists of Korea
2015 Volume.41 No. 3 p.287 ~ p.294
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Antioxidant and Cellular Protective Activities of Ecklonia cava Extracts against Reactive Oxyegen Species
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Yoo Cha-Young
Kim Si-Yun
Park Jung-Won
Sung Soo-An
Kim Da-Ae
Park Jee-Hyun
Xuan Song-Hua
Park Soo-Nam
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Abstract
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In this study, we investigated the antioxidative effects of brown seaweed Ecklonia cava extract and its subfractions. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction of E. cava. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities (FSC50) of ethyl acetate fraction (FSC50 = 6.98 μg/mL) and aglycone fraction (7.03 μg/mL) are similar to that of (+)-α-tocopherol (8.98 μg/mL) which is a reference control. Reactive oxygen species (ROS) scavenging activity (total antioxidant capacity, OSC50) of the aglycone fraction (OSC50 = 14.48 μg/mL) on ROS generated in Fe3+-EDTA/H2O2 system using the luminol-dependent chemiluminescence assay was the strongest among all extract and fractions. However, all samples showed lower anti-oxidant activities than that of L-ascorbic acid (6.88 μg/mL) known as a powerful antioxidant. The protective effect of 50% ethanol extract on the 1O2-induced cellular damage of human erythrocytes was dependent on the concentration from 5 to 50 μg/mL. Both ethyl acetate fraction and aglycone fraction showed strong cellular protective activities at 10 μg/mL, where the cellular protective effects (τ50) of each fraction were recorded 442.0 min and 539.9 min, respectively. Three kinds of extract/fractions of E. cava showed much greater cellular protective activities at 10 μg/mL than that of liposoluble antioxidant (+)-α-tocopherol (40.6 min) which is a reference control. These results suggest E. cava extracts and its fractions can be applied as an antioxidant ingredient in a field of cosmetics.
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KEYWORD
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antioxidant, reactive oxygen species, brown algae, Ecklonia cava, phlorotannin
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